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化学:论文文摘:
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1 | 微透析探针的制备及其在Ni(Ⅱ)与人血清白蛋白的相互作用研究中的应用 | Preparation of Microdialysis Probe and Its Application for the Study of Interaction of Ni(Ⅱ)with Human Serum Albumins | |
2 | 介绍了一种自制微透析探针的制备方法 | A method for the preparation of the microdialysis probe is reported. | |
3 | 实验结果表明:自制微透析探针透析金属离子的各种指标都与商品的微透析探针性能相当 | The self-made microdialysis probes showed characteristics similar to those of the commercial ones. | |
4 | 利用微透析与高效液相色谱联用技术研究了Ni(Ⅱ)与人血清白蛋白(HSA)的相互作用。 | The interaction of Ni(Ⅱ)with human serum albumin (HSA)was studied by these microdialysisprobes coupled with HPLC. | |
5 | 高效液相色谱法测定小鼠尿中的盐酸戊乙奎醚S型异构体 | Determination of the (S)-Isomers of Penehyclidine Hydrochloride in Mouse Urine by High Performance Liquid Chromatography | |
6 | 建立了测定动物体液中S型盐酸戊乙奎醚异构体的高效液相色谱法 | A high performance liquid chromatographic method was developed for the separation and determination of two (S)-isomers of penehyclidine hydrochloride (S-PHC: S-1, S-2)in mouse urine. | |
7 | S型异构体在小鼠尿中的比值(S-1\S-2)存在显著性差异。 | There was an obvious difference for the cumulative excretion of (S)-isomers in mouse urine after intramuscular injection of a single dose of S-PHC (30 μg). | |
8 | 48h内各异构体在尿中的排泄量明显不同,说明S型异构体在小鼠体内可能呈现立体选择性排泄。 | The results suggested that there could be the stereo-selective excretion to S-PHC isomers in mice. | |
9 | 用自制的微透析探针测定Ni(Ⅱ)与HSA之间的第一结合位点数和结合常数分别为1.16和8.6×105L\mol,采用商品的微透析探针测定的结果分别为1.23和7.5×105L\mol。 | With the self-made and commercial probes, the numberof primary binding sites of Ni(Ⅱ)on HSA was found to be 1.16 and 1.23, respectively, and the binding constant was found to be 8.6×105 L\mol and 7.5×105 L\mol, respectively. | |
10 | 此法简便、经济,可用于动物与人体中S型盐酸戊乙奎醚异构体代谢研究。 | The method is simple and low-cost and can be applied to study the excretion and metabolism of S-PHC in animals and human. | |
11 | 高效液相色谱法;盐酸戊乙奎醚;S型异构体;排泄 | high performance liquid chromatography; penehyclidine hydrochloride; (S)-isomers; excretion | |
12 | 高效液相色谱法快速测定血清中的芳香族氨基酸 | Rapid Analysis of Aromatic Amino Acids in Serum by High Performance Liquid Chromatography | |
13 | 采用高效液相色谱-紫外检测法分离测定了血清中的芳香族氨基酸。 | A high performance liquid chromatographic method, coupled with ultraviolet detection (HPLC-UV), was developed for the determination of aromatic amino acids in serum. | |
14 | 采用的色谱柱为Waters Nova-Pak C18柱(4μm,150mm×3.9mm i.d.),流动相为乙腈-水(体积比为6∶94,pH3.4)溶液,流速为1.0mL\min,检测波长为215nm。 | It employed a Waters Nova-Pak C18 column (4 μm, 3.9 mm i.d.×150 mm)and a mobile phase of acetonitrile-water (6∶94, v\v; pH 3.4)at a flow rate of 1.0 mL\min. | |
15 | 血清标本经5%(体积分数)高氯酸溶液去除蛋白质后取上清液直接进样,10min内完成测定。 | samples were first precipitated with a 5% perchloric acid solution, then centrifuged to remove protein residuals and finally assayed by HPLC with UV detection at 215 nm for 10 min. | |
16 | 该方法的日内、日间测定的相对标准偏差均小于5% | Interferences of other amino acids and dopamines were also investigated. Intraday and interday variations of the method were all within 5%. | |
17 | 芳香族氨基酸测定的线性范围、检测限、回收率等均满足临床检验的要求。 | All acceptance criteria forlinearity range, limit of detection and recovery were met in compliance with established clinical study requirements. | |
18 | 结果表明:该方法简便、快速、准确、可靠,适用于临床和科研工作。 | It has been demonstrated that the methodis simple, fast, accurate, and convenient, and suitable for routine. | |
19 | 高效液相色谱法;芳香族氨基酸;苯丙氨酸;酪氨酸;色氨酸;血清 | high performance liquid chromatography; aromatic amino acid; phenylalanine; tyrosine; tryptophan; serum | |
20 | 高效液相色谱法测定鸡肝中玉米赤霉醇的残留量 | Determination of Zeranol in Chicken Livers by High Performance Liquid Chromatography with Ultraviolet Detection |