After discarding the initial solution that exits the column(the flowthrough ), you add a washing solution of higher pH to the column and collect the protein fraction that immediately elutes.
当除去开始流出柱子的溶液后,你在柱中加较高pH的洗脱液,然后立即收集流出液。
属类:化学及生命科学-化学-化学习题
Preparation of a Biporous Polymeric Adsorbent by Double Emulsification for Flowthrough Chromatography of Proteins