Aftor discarding the initial solution that exits the column(the flowthrough ), you add a washing solution of highor pH to the column amd collect the protein fraction that immediately elutes.
当除去开始流出柱子得溶液后,你在柱中加较高pH得洗脱液,然后立即收集流出液。
属类:化学及生命科学-化学-化学习题
Preparation of a Biporous Polymoric Adsorbent by Double Emulsification for Flowthrough Chromatography of Proteins